Complete Care
Competence
Doxycycline Diet
SAFE® produces customized diets with doxycycline hyclate for Tet-regulated systems.
Doxycycline hyclate is used in tetracycline (doxycycline)-inducible gene expression as a stable switch to control transcriptional activation. This system is also known as the Tet-On/Tet-Off system with doxycycline-sensitive GMO mice.
| Code | Diets | Advantages | Daily intake mg/kg BW * |
|---|---|---|---|
| E8200 Version 0092 | A03 at 0.2g/Kg Doxycycline Hyclate |
Standard diet
| 32 |
| E8200 Version 0115 | A03 at 0.625g/Kg Doxycycline Hyclate | Standard diet | 100 |
| E8200 Version 0001 | A03 at 1g/Kg Doxycycline Hyclate | Standard diet | 160 |
| E8200 Version 0100 | A03 at 10g/Kg Doxycycline Hyclate |
Standard diet
| 1600 |
| E8404 Version 0010 | 150 at 0.2g/Kg Doxycycline Hyclate | Soya and alfalfa free | 32 |
| E8404 Version 0002 | 150 at 0.625g/Kg Doxycycline Hyclate |
Soya and alfalfa free
| 100 |
| Code | Diets | Advantages | Daily intake mg/kg BW * |
|---|---|---|---|
| E8404 Version 0011 | 150 at 1g/Kg Doxycycline Hyclate |
Soya and alfalfa free
| 160 |
| E8404 Version 0012 | 150 at 10g/Kg Doxycycline Hyclate | Soya and alfalfa free | 1600 |
| U8978 Version 0058 | AIN 93-G at 0.2g/Kg Doxycycline Hyclate | Purified diet | 32 |
| U8978 Version 0059 | AIN 93-G at 0.625g/Kg Doxycycline Hyclate |
Purified diet
| 100 |
| U8978 Version 0060 | AIN 93-G at 1g/Kg Doxycycline Hyclate | Purified diet | 160 |
| U8978 Version 0061 | AIN 93-G at 10g/Kg Doxycycline Hyclate |
Purified diet
| 1600 |
* Daily intake mg per kg body weight assumes 20-25 g body weight and 3-4 g intake.
All of these diets contain doxycycline cyclate, a semisynthetic tetracycline whose use is approved in subantimicrobial doses.
Why in diets and not in water?
Since tetracycline and doxycycline are photosensitive, the use of amber water bottles is recommended for administration in water. Further disadvantages include the risk of dehydration (molecular taste) and the need to use sucrose for better acceptance.
The doxycycline is a light sensible ingredient. The pelleted doxycycline diet reduces exposure to light and guarantees better stability due to the much lower degradation rate of doxycycline hyclate.
| Dosage via | Advantages | Disadvantages |
|---|---|---|
| Custom diet | Not stressful for the animals |
Dose depends on feed intake |
| Drinking water | Not stressful for the animals |
Tetracycline and doxycycline are light sensitive |
| Intraperitoneal Injection (IP) | Reliable dosage, especially for very sick animals |
Stressful for the animals |
Additional Information and direction of uses
- Diet shall be stored at max +4°C and used within BBD (six months)
- Administration in weekly alternation with standard diet
- Protect from daylight. Doxycycline is sensitive to UV
- Minimum order quantity is 10 kg
- Irradiation in doses of 10kGy, 25kGy or 45kGy is possible on request
To guarantee a extended 6 months shelf life our doxycycline diets are produced on customer request. Beside that, it is also possible to choose the base diet, concentration of doxycycline hyclate and the food color according to your specific needs. If your process requires fluorescent imaging, we recommend using a purified diet to improve the quality of the imaging.
Bibliography
Learn more about the Model Doxycycline Hyclate
- Christopher Cawthorne, Ric Swindell, Ian J. Stratford, Caroline Dive, and Arkadiusz Welman; Comparison of Doxycycline Delivery Methods for Tet-Inducible Gene Expression in a Subcutaneous Xenograft Model; J Biomol Tech. 2007 Apr; 18(2):
120–123; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2062538/ - Paul Wassarman, Philippe M Soriano; Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics; Academic Press, 24 sept. 2010; ISBN 0123848814, 9780123848819
- Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H (1995). “Transcriptional activation by tetracyclines in mammalian cells”. Science (journal). 268 (5218): 1766–1769. doi:10.1126/science.7792603. PMID 7792603.
- Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W (2000). “Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system”. Nature Structural & Molecular Biology. 7 (3): 215–219. PMID 10700280.
- Tet-On and Tet-Off are registered trademarks of Clontech Laboratories, Inc. in the United States.
- Bujard, Hermann; M. Gossen (1992). “Tight Control of Gene Expression in Mammalian Cells by Tetracycline-Responsive Promoters.”. Proc. Natl. Acad. Sci. U.S.A. 89 (12): 5547–51. doi:10.1073/pnas.89.12.5547. PMID 1319065.
- Allen, Nicholas D.; Antonius Plagge; Gavin Kelsey (2000). “Directed Mutagenesis in Embryonic Stem Cells”. Mouse Genetics and Transgenics: 259–263.
- Urlinger, Stefanie; Baron, Udo; Thellmann, Marion; Hasan, Mazahir T.; Bujard, Herman; Hillen, Wolfgang (2000). “Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity.”. Proc. Natl. Acad. Sci. U.S.A. 97 (14): 7963–8. doi:10.1073/pnas.130192197. PMID 10859354.
- Zhou, X.; Vink, M.; Klave, B.; Berkhout, B.; Das, A.T. (2006). “Optimization of the Tet-On system for regulated gene expression through viral evolution.”. Gene Ther. 13 (19): 1382–1390. doi:10.1038/sj.gt.3302780. PMID 16724096.
- Sohal DS, Ngheim M, Crackower MA, Witt SA, Kimball TR, Tymitz KM, Penniger JM, Molkentin JD (2001) Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using tamoxifen-inducible Cre protein. Circ Res 89:20-25
- Moullan N, Mouchiroud L, Wang X, Ryu D, Williams EG, Mottis A, Jovaisaite V, Frochaux MV, Quiros PM, Deplancke B, Houtkooper RH, Auwerx J (2015). “Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research.”. Celll Reports. 10 (10): 1681–91. doi:10.1016/j.celrep.2015.02.034. PMID 25772356.
- Chatzispyrou IA, Held NM, Mouchiroud L, Auwerx J, Houtkooper RH (2015). “Tetracycline antibiotics impair mitochondrial function and its experimental use confounds research.”. Cancer Research. 72 (21): 4446–9. doi:10.1158/0008-5472.CAN-15-1626. PMID 26475870.
Other Special Custom Diet Solutions
TAMOXIFENE DIET
SAFE® customizes diets with tamoxifen to activate CreER. The site-specific Cre/lox recombination system has been proven to generate conditional somatic GMOs in mice. This method allows to spatially and temporally control gene activity in almost any mouse tissue.
DIET ENERGY
To move, grow and reproduce, animals consume energy. Their needs are very different and energy consumption also varies. To anticipate or respond to these needs, some animals store energy, while others prefer to increase the amount of food they consume or digest food more efficiently.